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Assessment of the tube formation capability, CD31 and <t>VEGF</t> gene expressions for HUVECs treated without (control) and with cell culture supernatant collected <t>from</t> <t>H-EMSCs</t> seeded on PCL-HA (PCL-HA/H-E) or H-EMSCs and mø co-seeded on PCL-HA (PCL-HA/H-E/mø). A Tube formation assay of HUVECs in the control, PCL-HA/H-E and PCL-HA/H-E/mø groups. Scale bar = 200 μm. B Quantification of the total length of the tubes formed. C Quantification of the total number of meshes formed. D Quantification of the number of nodes formed. E CD31 gene expression of the HUVECs. F VEGF gene expression of the HUVECs. *** p < 0.001. ## p < 0.01, ### p < 0.001 vs. Control
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Assessment of the tube formation capability, CD31 and VEGF gene expressions for HUVECs treated without (control) and with cell culture supernatant collected from H-EMSCs seeded on PCL-HA (PCL-HA/H-E) or H-EMSCs and mø co-seeded on PCL-HA (PCL-HA/H-E/mø). A Tube formation assay of HUVECs in the control, PCL-HA/H-E and PCL-HA/H-E/mø groups. Scale bar = 200 μm. B Quantification of the total length of the tubes formed. C Quantification of the total number of meshes formed. D Quantification of the number of nodes formed. E CD31 gene expression of the HUVECs. F VEGF gene expression of the HUVECs. *** p < 0.001. ## p < 0.01, ### p < 0.001 vs. Control

Journal: Stem Cell Research & Therapy

Article Title: Co-delivery of endometrial mesenchymal stem cells and macrophages by an electrospun patch promotes angiogenesis during endometrial injury repair via VEGF related signalling

doi: 10.1186/s13287-026-04929-2

Figure Lengend Snippet: Assessment of the tube formation capability, CD31 and VEGF gene expressions for HUVECs treated without (control) and with cell culture supernatant collected from H-EMSCs seeded on PCL-HA (PCL-HA/H-E) or H-EMSCs and mø co-seeded on PCL-HA (PCL-HA/H-E/mø). A Tube formation assay of HUVECs in the control, PCL-HA/H-E and PCL-HA/H-E/mø groups. Scale bar = 200 μm. B Quantification of the total length of the tubes formed. C Quantification of the total number of meshes formed. D Quantification of the number of nodes formed. E CD31 gene expression of the HUVECs. F VEGF gene expression of the HUVECs. *** p < 0.001. ## p < 0.01, ### p < 0.001 vs. Control

Article Snippet: For the VEGF inhibition experiments, 18 female SD rats in estrus were divided into the following three groups, with 6 rats in each group: PCL-HA/H-E group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs) was transplanted; PCL-HA/H-E/mø group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs + 0.25 × 10 6 mø) was transplanted; PCL-HA/H-E/mø/VEGF inhibitor group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs and 0.25 × 10 6 mø) was implanted, VEGF inhibitor (Axitinib (HY-10065, MCE), 100 mg/kg) was injected into the rat uterine cavity.

Techniques: Control, Cell Culture, Tube Formation Assay, Gene Expression

Assessment of the tube formation capability, CD31 and VEGF gene expressions of HUVECs treated with cell culture supernatant collected from H-EMSCs seeded on PCL-HA (PCL-HA/H-E) or H-EMSCs and mø co-seeded on PCL-HA (PCL-HA/H-E/mø) or H-EMSCs and mø co-seeded on PCL-HA and VEGF inhibitor (PCL-HA/H-E/mø/VEGF inhibitor). A Tube formation assay of HUVECs in the PCL-HA/H-E, PCL-HA/H-E/mø and PCL-HA/H-E/mø/VEGF inhibitor groups. Scale bar = 200 μm. B Quantification of the total length of the tubes formed. C Quantification of the total number of meshes formed. D Quantification of the number of nodes formed. E CD31 gene expression of the HUVECs. F VEGF gene expression of the HUVECs. *** p < 0.001. # p < 0.05, ### p < 0.001 vs. PCL-HA/H-E group

Journal: Stem Cell Research & Therapy

Article Title: Co-delivery of endometrial mesenchymal stem cells and macrophages by an electrospun patch promotes angiogenesis during endometrial injury repair via VEGF related signalling

doi: 10.1186/s13287-026-04929-2

Figure Lengend Snippet: Assessment of the tube formation capability, CD31 and VEGF gene expressions of HUVECs treated with cell culture supernatant collected from H-EMSCs seeded on PCL-HA (PCL-HA/H-E) or H-EMSCs and mø co-seeded on PCL-HA (PCL-HA/H-E/mø) or H-EMSCs and mø co-seeded on PCL-HA and VEGF inhibitor (PCL-HA/H-E/mø/VEGF inhibitor). A Tube formation assay of HUVECs in the PCL-HA/H-E, PCL-HA/H-E/mø and PCL-HA/H-E/mø/VEGF inhibitor groups. Scale bar = 200 μm. B Quantification of the total length of the tubes formed. C Quantification of the total number of meshes formed. D Quantification of the number of nodes formed. E CD31 gene expression of the HUVECs. F VEGF gene expression of the HUVECs. *** p < 0.001. # p < 0.05, ### p < 0.001 vs. PCL-HA/H-E group

Article Snippet: For the VEGF inhibition experiments, 18 female SD rats in estrus were divided into the following three groups, with 6 rats in each group: PCL-HA/H-E group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs) was transplanted; PCL-HA/H-E/mø group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs + 0.25 × 10 6 mø) was transplanted; PCL-HA/H-E/mø/VEGF inhibitor group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs and 0.25 × 10 6 mø) was implanted, VEGF inhibitor (Axitinib (HY-10065, MCE), 100 mg/kg) was injected into the rat uterine cavity.

Techniques: Cell Culture, Tube Formation Assay, Gene Expression

VEGF, CD31 gene and protein expression of rat endometrial tissues of the PCL-HA/H-E, PCL-HA/H-E/mø and PCL-HA/H-E/mø/VEGF inhibitor groups at day 7. A VEGF gene expression. B Quantification of VEGF protein concentration (pg/ml). C Representative images of VEGF immunostaining. Cell nuclei stained in blue while VEGF stained in red. Scale bar = 100 μm. D Quantification of VEGF staining area (%). E CD31 gene expression. F Representative images of CD31 immunostaining. Cell nuclei stained in blue while CD31 stained in red. Scale bar = 100 μm. G Quantification of CD31 staining area (%). *** p < 0.001. ## p < 0.01, ### p < 0.001 vs. PCL-HA/H-E group

Journal: Stem Cell Research & Therapy

Article Title: Co-delivery of endometrial mesenchymal stem cells and macrophages by an electrospun patch promotes angiogenesis during endometrial injury repair via VEGF related signalling

doi: 10.1186/s13287-026-04929-2

Figure Lengend Snippet: VEGF, CD31 gene and protein expression of rat endometrial tissues of the PCL-HA/H-E, PCL-HA/H-E/mø and PCL-HA/H-E/mø/VEGF inhibitor groups at day 7. A VEGF gene expression. B Quantification of VEGF protein concentration (pg/ml). C Representative images of VEGF immunostaining. Cell nuclei stained in blue while VEGF stained in red. Scale bar = 100 μm. D Quantification of VEGF staining area (%). E CD31 gene expression. F Representative images of CD31 immunostaining. Cell nuclei stained in blue while CD31 stained in red. Scale bar = 100 μm. G Quantification of CD31 staining area (%). *** p < 0.001. ## p < 0.01, ### p < 0.001 vs. PCL-HA/H-E group

Article Snippet: For the VEGF inhibition experiments, 18 female SD rats in estrus were divided into the following three groups, with 6 rats in each group: PCL-HA/H-E group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs) was transplanted; PCL-HA/H-E/mø group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs + 0.25 × 10 6 mø) was transplanted; PCL-HA/H-E/mø/VEGF inhibitor group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs and 0.25 × 10 6 mø) was implanted, VEGF inhibitor (Axitinib (HY-10065, MCE), 100 mg/kg) was injected into the rat uterine cavity.

Techniques: Expressing, Gene Expression, Protein Concentration, Immunostaining, Staining

H&E staining of rat endometrial tissues of the following groups: PCL-HA carrier with H-EMSCs mono-delivery (PCL-HA/H-E), PCL-HA carrier with H-EMSCs and mø co-delivery (PCL-HA/H-E/mø) and PCL-HA/H-E/mø with VEGF inhibitor (PCL-HA/H-E/mø/VEGF inhibitor), at day 7. A Representative H&E images of the rat endometrial tissues of the different treatment groups at day 7. Scale bar = 560 μm. Enlarged images are shown at the bottom and scale bar = 220 μm. B Quantification of the endometrium thickness. C Quantification of the number of endometrial glands. *** p < 0.001, ## p < 0.01, ### p < 0.001 vs. PCL-HA/H-E group

Journal: Stem Cell Research & Therapy

Article Title: Co-delivery of endometrial mesenchymal stem cells and macrophages by an electrospun patch promotes angiogenesis during endometrial injury repair via VEGF related signalling

doi: 10.1186/s13287-026-04929-2

Figure Lengend Snippet: H&E staining of rat endometrial tissues of the following groups: PCL-HA carrier with H-EMSCs mono-delivery (PCL-HA/H-E), PCL-HA carrier with H-EMSCs and mø co-delivery (PCL-HA/H-E/mø) and PCL-HA/H-E/mø with VEGF inhibitor (PCL-HA/H-E/mø/VEGF inhibitor), at day 7. A Representative H&E images of the rat endometrial tissues of the different treatment groups at day 7. Scale bar = 560 μm. Enlarged images are shown at the bottom and scale bar = 220 μm. B Quantification of the endometrium thickness. C Quantification of the number of endometrial glands. *** p < 0.001, ## p < 0.01, ### p < 0.001 vs. PCL-HA/H-E group

Article Snippet: For the VEGF inhibition experiments, 18 female SD rats in estrus were divided into the following three groups, with 6 rats in each group: PCL-HA/H-E group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs) was transplanted; PCL-HA/H-E/mø group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs + 0.25 × 10 6 mø) was transplanted; PCL-HA/H-E/mø/VEGF inhibitor group: PCL-HA electrospun carrier (1 × 10 6 H-EMSCs and 0.25 × 10 6 mø) was implanted, VEGF inhibitor (Axitinib (HY-10065, MCE), 100 mg/kg) was injected into the rat uterine cavity.

Techniques: Staining